Dirk is currently a PhD student with Prof Andrew deMello in the Institute for Chemical and Bioengineering at ETH Zurich. He has an MEng in Bioengineering from Imperial College London, with the first two years spent taking engineering and medical science courses in the Bioengineering Department, and the third year in the Electrical Engineering Department. His final undergraduate year was spent abroad at University of California, Berkeley, where he worked with Prof Luke Lee in the Bioengineering Department and took classes in BioMEMS, microfluidics, robotics, and molecular biomechanics.
We present a novel connector that allows for easy handling and injection of sample volumes between 1 and 20 μl. All tubing connections between external pumps and the microfluidic device are established before the sample is introduced into a sealable reservoir built into the connector. This approach allows for multiple injections of small sample volumes without the need to dismantle the chip-tubing assembly. We demonstrate that the connector reservoir seal can withstand pressures of up to 6 bar, that opening or closing the reservoir does not dislocate the sample by more than 35 nl, and that the connector can be used for injecting samples into both miscible and immiscible carrier fluids.
Liposome structures have a wide range of applications in biology, biochemistry, and biophysics. As a result, several methods for forming liposomes have been developed. This review provides a critical comparison of existing microfluidic technologies for forming liposomes and, when applicable, a comparison with their analogous macroscale counterparts. The properties of the generated liposomes, including size, size distribution, lamellarity, membrane composition, and encapsulation efficiency, form the basis for comparison. We hope that this critique will allow the reader to make an informed decision as to which method should be used for a given biological application.