High-Throughput Imaging of Cells Using Microlenses

A novel microfluidic system uses microlenses for simultaneous imaging of cells in multiple channels

Gregor Holzner and coworkers from the deMello group have devised a novel method for imaging flowing cells in a microfluidic chip using a microlens array. The combination of microlenses, aligned with multiple parallel channels, and a low-magnifying objective enabled simultaneous high-resolution imaging of cells flowing in separate channels. The system successfully achieved a throughput of 50,000 cells per second and can help significantly in the advancement of flow cytometry, a technique used for analysing the physical and chemical characteristics of cells in flow.

Microfluidic systems often require high-magnification objectives to resolve sub-cellular features. These high magnification objectives have a smaller field of view, imaging only a small area of the microfluidic channel. Although low-magnification objectives have a larger field of view, they cannot resolve sub-cellular fea